Study of the regulation of proliferative activity and trophoblast cell death

Abstract

Damage to the surface of the trophoblast induces stimulation of proliferative processes. Researchers suggest that iron deficiency anaemia in pregnancy (IDAP) leads to hypoxia and, consequently, to alterations in the trophoblast surface. Aim of the study. To determine the adaptive and compensatory mechanisms of the trophoblast in response to chronic hypoxia. Material and methods. The study design consisted of two main groups and a control group. A series of immunohistochemical reactions was performed using mono- and polyclonal antibodies (DakoCytomation, Denmark–USA): pro-apoptotic protein Bax (Polyclonal Rabbit Anti-Human Bax); anti-apoptotic protein Bcl-2 (Monoclonal Mouse Anti-Human BCL2, Clone 124); proliferative nuclear antigen Ki-67 (Monoclonal Mouse Anti-Human MIB-1). Statistical significance was determined at p ≤ 0.05.