Features of the morphofunctional state of endothelial cells of the cortex of the great hemispheres in rats with streptozotocin- induced diabetes under the conditions of ischemic-reperfusion brain damage

Abstract

Diabetes mellitus (DM) is one of the leading medical and social problems of our time. In Ukraine, more than 1 million patients are registered, but the real number of people with undiagnosed forms is several times higher. The main causes of mortality in DM remain cardiovascular complications, in particular cerebral circulation disorders. Endothelial dysfunction is considered the earliest manifestation of vascular damage in diabetes and ischemic-reperfusion brain damage. At the same time, data on the reaction of endothelial cells of different areas of the cerebral cortex to a combination of both pathological factors are limited. The aim of the study. To study the selective sensitivity of endothelial cells of the auditory and sensory cortex of the rat brain to incomplete global ischemia-reperfusion in the setting of streptozotocin-induced diabetes. Material and methods. The experiments were conducted on 6-month-old male rats, which were divided into 4 groups: 1. Control; 2. Rats with diabetes; 3. Animals with diabetes, who were simulated 20-minute bilateral carotid ischemia-reperfusion (BCIR) with one-hour reperfusion; 4. Rats with diabetes, who were withdrawn from the experiment on the 12th day after the simulation of 20-minute BCIR. Brain tissue was fixed in Bouin’s solution, embedded in paraffin, and serial sections with a thickness of 5 μm were prepared. Morphometry was performed using the VIDAS-2.5 system (Kontron Elektronik, Germany). The density, equivalent diameter and RNA concentration of endothelial cells were determined.