The value of pyruvate in the energy metabolism of rats with alloxan diabetes under the conditions of melatonin administration

Abstract

It is known that pyruvate is an intermediate product connecting glycolysis and the cycle of tricarboxylic acids with the subsequent formation of ATP, in addition, it can be used in the formation of essential amino acids and fatty acids. Melatonin is a hormone widely known as an antioxidant and cytoprotector. Exactly how pyruvate concentration changes under conditions of diabetes and melatonin administration is still poorly understood. The aim of the study. To find out the effect of melatonin on the level of pyruvate in the plasma of rats with alloxan diabetes. Materials and methods. For the experiment, 60 sexually mature outbred male rats weighing 150 grams were selected. The animals were divided into 4 groups: 1) control, 2) control + melatonin 10 mg/kg body weight, 3) alloxan diabetic rats (alloxan monohydrate intraperitoneally at the rate of 170 mg/kg body weight), 4) alloxan diabetic rats that received melatonin similarly. Fasting glucose level was determined using the One Touch Ultra Easy (Johnson & Johnson, USA). Rats were killed by decapitation under light ether anesthesia on the 12th day from the beginning of the experiment in accordance with the established norms for the treatment of experimental animals. The level of pyruvate in the blood plasma was measured according to the standard method. Statistical processing of the research results was carried out using the Student's t-test.