Catalase activity in the blood and liver of rats by experimental nephropathy and influence of glutathione

Abstract

Under the conditions of entry of toxic substances into the body and activation of redox processes, compounds of natural origin with antioxidant properties are widely used to correct metabolic disorders. Among water-soluble antioxidants, low- and high-molecular compounds containing SH-groups are popular in medicine, mono-, di- and tricarboxylic acids and other anions. Glutathione, a thiol-containing tripeptide, is the main endogenous non-enzymatic antioxidant that exerts cytoprotective and detoxifying properties. The function of the glutathione system affects the implementation of important physiological and biochemical processes: detoxification, antioxidant protection, transformation vitamins C and E, lipoic acid, ubiquinone, regulation of thiol disulfide balance, synthesis of nucleic acids, preservation of the optimal state and functions of biological membranes, participates in the exchange of eicosanoids - prostaglandins and leukotrienes. Oxidative modification or protein carbonylation, which occurs at depletion of glutathione, stimulates covalent modification of endogenous enzymes and proteins, which can lead to loss of their functions. In the kidneys, there are peculiarities of glutathione metabolism.Therefore, disorders of the structure of the kidneys, diseases of the excretory system require additional studies of glutathione metabolism and antioxidants enzymes. The aim of the study. To determine catalase activity in the blood and liver of rats by experimental nephropathy and influence of glutathione. Material and methods. The experiment was carried out on 131 male albino rats with the bodyweight of 0,16–0,18 kg. Experimental nephropathy was modeled by injection of a single intraperitoneal dose of folic acid (250 mg/kg, Sigma-Aldrich). Glutathione (Sigma-Aldrich) was introduced daily (100 mg/kg) by the intragastric way for 3 and 7 days following the injection of folic acid.