Effectiveness of autologous mesoconcentrate (PRGF) in clinical dentistry: electron microscopic analysis

Abstract

Abstract. This paper provides a scientific assessment of a widely implemented technology in regenerative medicine, particularly clinical dentistry, subject to protocol compliance with the method of ordering fibrin filaments, which ensures the formation of a three-dimensional structure that serves as a biological scaffold with multifunctional properties. The preservation of autologous genetic information with full stimulating content, with a spectrum of active trophic and repair factors – growth factors, in particular, of platelet origin, provides a reliable prognosis of “controlled” tissue regeneration. The aim of the study is to substantiate the effectiveness of the use of autologous plasma products – plasma rich in growth factors (PRGF) in clinical dentistry, and their interfractional differentiation according to the results of electron microscopic analysis. We have studied the density of formed fibrin fibers in terms of the number (in 10 μm2) and morphometric value of the cross-section (Ø) in the PRGF – F1 and F2 fractions of mesoconcentrate products, namely, insulating membranes (M) and wrapping blocks (B), using electron transmission microscopy. For evidentiary analytical substantiation, we performed scanning electron microscopy of fibrin strands, which provided high-resolution images of sample surfaces without destroying their architectonics. The obtained results confirm the working hypothesis, i.e., the acceptable effectiveness of the study based on statistical analysis. This highlights the reliability of the results with the appropriate level of significance in the interfraction difference of PRGF F1-M, B and F2-M, B, with a description of the interrelated diametric differences. This analysis of the accumulation and quality of fibrin filament formation based on the results of electron microscopic examination, comprehensively confirmed by scanning electron microscopy and in the above fragment of clinical application, gives priority to the PRGF method with targeted fractional use (F1, F2) of mesoconcentrate products for targeted tissue regeneration, in particular in clinical dentistry according to its extensive indications.