Caspase-8 and caspase-3 activity in lymphocyte lysate of patients with chronic obstructive pulmonary disease and intestinal dysbiosis

Abstract

With the aim to define the role of lymphocyte apoptosis in the mechanisms of T-cell deficiency in patients with chronic obstructive pulmonary disease (COPD) accompanied by the disturbances of intestinal microbiocynosis, the investigation of caspases activity in lysate of lymphocytes was conducted. III degree of dysbiosis was found to be followed by the reduction of CD3+- and CD8+-cells number, and in patients with IV degree of intestinal microbiocynosis the level of lymphocytes of CD3+, CD4+, CD8+ phenotypes decreased in the peripheral blood, followed by the inhibition of phytohaemagglutinin (PHA) induced lymphocyte blast transformation. The activity of caspase-8, caspase-3 and caspase-1 in lymphocyte lysate of patients with COPD was of high rates in dysbiosis of III and IV degree.