Biosensors for Cellular Imaging on the Base of Colloidal CdMnS Nanoparticles

Abstract

Semiconductor nanoparticles have rapidly found a broad range of applications as optical imaging agents. The presented paper describes the growth, analysis of optical spectra and study of cellular imaging of Mn-doped cadmium sulfide nanoparticles. Semimagnetic semiconductor nanoparticles CdMnS are characterized by high brightness, improved photostability and multicolor size- and structure-dependent light emission in the visible range of spectra. They are emerging as a new class of fluorescent reporters with properties and applications that are not available with traditional organic dyes. In order to obtain nanosensors different surfactants, growth factors and conditions such as surfactant concentrations, ionic strength, precursor concentration have been varied. Formation of nanoparticles was monitored by optical methods. The average nanoparticle size was estimated by optical spectra. For cellular research histological section of a placental tissues and liver tissues has been used. This procedure is possible only after cell fixation and permeabilization treatment is needed to allow the nanosensors to enter inside the cell.