The study of enalapril effect on the functional-metabolic parameters of the cerebral mitochondria in rats with type 2 diabetes mellitus

Abstract

Abstract. The results of experimental and clinical studies do not exclude interrelations between diabetes mellitus and local renin-angiotensin system of the brain. Considering an important role of the system in development of neurodegenerative processes we have become interested in the issue concerning enalapril effect on the mitochondrial state of the cerebral cortex and hippocampus in experimental simulation of type 2 diabetes mellitus. Objective of the study is to learn enalapril effect on the functional-metabolic parameters of mitochondria in the cerebral cortex and hippocampus of rats under neurodegeneration conditions stipulated by type 2 diabetes mellitus. The experiments were conducted on laboratory nonlinear albino male rats with the body weight 0.18-0.20 kg. Type 2 diabetes is modeled on streptozotocin and a high-fat diet. Enalapril was administered intraperitoneally at a dose of 1 mg/kg, once daily for 14 days. The administration of 14 days of enalapril was found in the dose of 1 mg/kg during 14 days produces a positive effect on the functional-metabolic state of the mitochondria in the cerebral cortex and hippocampus in rats with type 2 diabetes mellitus, which is indicative of decreased relative rate of mitochondrial swelling and increased intensity of light dispersion, and increased activity of α-ketoglutarate dehydrogenase and succinate dehydrogenase. The use of enalapril rats with type 2 diabetes improves the state of the prooxidant-antioxidant system in the cerebral cortex and hippocampus by the degree of reduced markers of protein and lipid modification oxidation (reduced content of carboxyl phenylhydrazine and products reacting with 2-thiobarbituric acid) and increased catalase activity. The data obtained present experimental substantiation of enalapril protective effects in case of mitochondrial dysfunction in the cerebral cortex and hippocampus of rats induced by type 2 diabetes mellitus.