Comparative characteristics of different methods for diagnosis of Helicobacter pylori in children

Abstract

Abstract. Comparative evaluation of the results of different methods of infection H. pylori is shown. The study involved 120 patients 7-18 years old with the pathology of the upper gastrointestinal tract. Fibrogastroduodenoscopy was conducted with fence biopsies from the body, antrum and duodenum bulb. Verification of H. pylori was performed by the following methods: express methods (industrial sets "CLO-test" the company "Delta" (Australia), "De-Nol-test" firm "Yamanouchi" (Japan)) and laboratory tests ("Campy-test" (Russia), "Helikotest" (Russia) and rapid urease test (Russia)), histological investigation, PCR detection ureC, CagA, VacA gene pathogenicity islands microorganism, biological, enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin classes M, A and G to CagA H. pylori antigen in serum. There was established that all express tests have a high sensitivity and specificity. To improve the accuracy of diagnosis helicobacter infection it is recommended to use at least two, and preferably three, research methods, preferably a combination of express urease test or "De-Nol-test" with histological methods (biopsy of the antrum of the stomach) or PCR (gene detection ureC, CagA and VacA). "Helic test" is recommended as an exact noninvasive method for assessing the effectiveness of eradication therapy, especially by children.