Effect of pro197leu polymorphism of the gene GPX 1 on functional state of endothelium indices of patients with metabolic syndrome

Abstract

Pro197Leu polymorphism of the gene GPX 1 has been studied in 102 patients with MS and 97 healthy individuals. Disorders of genotype frequencies distribution comparing with the control group at the expense of the reduction of Pro/Pro genotype frequency have been found in the main group (ð < 0,05). Assessment of the relative risk was carried by odds ratio (OR) magnitude. The OR calculation showed the growth of risk of GPX 1 activity disorder in patients with Pro/Leu and Leu/Leu variants of polymorphism comparing with homozygous for the Pro-allele at 4,7 and 6,9 times respectively (ð < 0,05). Individuals with Leu/Leu genotype had significantly higher level of VEGF and endothelial desquamation intensity as compared with the persons with Pro/Pro genotype (ð < 0,05). Consequently, Pro-allele has protective properties, it means that presence of these allele in genotype reduses risk of glutathione peroxidase activity decreasing. In Leu-allele carriers, activity of GPX 1 decreases, free radicals accumulate, that cause lipid peroxidation and activation of transcription factors, followed by increasing expression of cytokines, which are also directly affect the endothelium.