Experimental study of colon preepithelial mucous layer microflora in albino rats with induced diabetes mellitus

Abstract

The aim of research. This experimental issue deals with microbiological investigation of quantitative and qualitative composition of microflora of preepithelium mucous layer of colon in albino rats with aloxan-induced diabetes mellitus. Methods. Case-control experimental study conducted on white 20 albino rats with weight from 200 to 220 g: 10 intact animals (control group) and 10 rats in basic group with aloxan-stimulated diabetes mellitus. The pieces of colon served as research material for microbiological investigation with obtaining pure cultures of microorganisms. Population level of microflora of pre-epithelium mucous layer of colon was displayed in logarithm of colony-forming units (lg CFU/g). Statistical analysis was performed by Student’s t-test. Results. Aloxan-induced diabetes mellitus in experimental animals leads to abnormalities of quantitative composition – the rising of constancy index (CI) of species that related to additional and residual microbiota: E.coli Hly+ was isolated with CI 30%, Clostridium – 70%, and Staphylococcus, Proteus, Peptococcus – 40%. Qualitative abnormalities include decrease of Bifidobacteria populational level – 5.60±0.29 vs 6.65±0.27 lgCFU/g (p<0.05), Bacteroides – 2.21±0.15 vs 6.50±0.18 lgCFU/g (p<0.001), E.coli – 2.25±0.16 vs 5.77±0.19 lgCFU/g (p<0.001) and Enterococcus 2.08±0.07 vs 6.17±0.22 lgCFU/g (p<0.01). Meanwhile Peptostreptococ-cus populational level had moderate increase to 4.13±0.19 vs 3.22±0.19 lgCFU/g (p<0.05). Conclusions. In basic group the pre-epithelial mucous layer of colon was contaminated with opportunistic pathogenic Peptococcus, Clostridium, Proteus, Staphylococcus. Autochtonous obligatory Bifidobacteria, Lactobacilli, Bacteroides remain dominant role, only Eubacterium was completely eliminated. It probably had great impact on the further weakness of colonization resistance of gut, accom¬panying diabetes in animal model.