The effect of the nucleinat on functional activity blood granulocytes in school-age children with asthma, depending on acetylation phenotype

Abstract

Introduction. Bronchial asthma is one of the most common diseases in the world, the number of patient with an ever increasing, especially among children. Inadequate efficiency of controlling asthma therapy necessitated the application of new drugs in children which have improved the course of the disease. Objective. To assess the Nucleinat effect in the treatment of asthma on indicators of the functional activity of blood granulocytes school-age children depend of their atsetylyatornyh phenotypes. Materials and Methods. 36 school-age children with bronchial asthma in remission were complex examined. All pupils in the complex basic therapy received Nucleinat 0,25 g per day for 21 days. Due to the type of acetylation patients were divided into two clinical groups. The first clinical group included 16 patients who determined the slow type of acetylation (average percentage acetylated sulfadimezin of urine was less than 75,0%). 20 students formed the second clinical group of, which is marked the rapid nature of acetylation (average percentage acetylated sulfadimezin urine was more than 75,0%). All children conducted by determining phagocytic activity and phagocytic number of blood neutrophil, analis of oxygen-dependent metabolism of eosinophilic and neutrophilic blood granulocytes due to parameters of spontaneous and stimulated Nitroblue tetrazolium test.

Results. This definition of relative risk above the absolute number of neutrophils in farmazan positive spontaneous NBT-test less than 0,42 S. U. in pupils with the slow type of acetylation after therapy was 1,9 (95% CI 1,6-2,4) and absolute risk – 0,3. The using of Nucleinat with basic anti-inflammatory therapy in children with rapid type of acetylation compared to slow type of acetylation, accompanied by the risk of inadequate asthma control, namely the ability to increase the activity of the immune system (OR = 3,3, 95% CI 1,7-6,4).