ASSOCIATION OF A313G POLYMORPHISM OF GSTP1 GENE WITH BIOCHEMICAL BLOOD PARAMETERS IN PATIENTS WITH NONALCOHOLIC FATTY LIVER DISEASE

Abstract

Background: One of the key gene involved in chronic diffuse liver diseases pathogenesis, including nonalcoholic fatty liver disease are genes encoding the synthesis of glutathione-S-transferases. The objective of the study was to investigate a possible link between A313G polymorphism of the GSTP1 gene and biochemical blood parameters in nonalcoholic fatty liver disease patients. Methods: Gene polymorphism of GSTP1 (A313G) in 64 non-alcoholic fatty liver disease patients and 20 healthy individuals (control group) was studied. The range of investigated biochemical blood parameters included: total bilirubin and its fractions, cholesterol, triglycerides, uric acid, total protein and albumin, urea, creatinine, plasma enzyme activity. Results: G-allele significantly more frequently by 2.47 times (OR = 2.47, CI = 1.01-6.03, p<0.05) occurred in observed patients compared with healthy individuals. Total bilirubin level in blood of patients with GG-genotype was by 19.7% (p = 0.03) and by 36.9% (p = 0.04) higher compared with AA-genotype and AG-genotype carriers respectively. Presence of G-allele was associated with increased alanine aminotransferase activity, which was significantly higher in observed patients AG- and GG-genotypes carriers compared to patients with-AA genotype at 60.7% (p =0.03) and 51.0% (p = 0.04) respectively. Conclusions: Frequency of G-allele of GSTP1 gene was in 2.47 times (OR = 2.47, CI = 1.01-6.03, p<0.05) higher in nonalcoholic fatty liver disease patients compared with healthy individuals in Ukrainian population. Presence of G-allele was associated with increased concentration of total bilirubin and higher activity of cytolytic syndrome in observed patients.